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cmv promoter driven acriia4 expression vectors  (Addgene inc)


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    Addgene inc cmv promoter driven acriia4 expression vectors
    AcrIIA11 inhibits SaCas9 in vitro and in human HEK293T cells. ( A ) Agarose gel and ( B ) quantification of DNA cleavage by SaCas9. Graphs represent the mean of three replicates. Error bars: S.E.M. ( C ) HEK293Ts are transiently transfected with plasmids carrying SaCas9 + sgRNA. A second plasmid encodes either <t>AcrIIA4</t> (positive control) or AcrIIA11. ( D ) Representative agarose gel and ( E ) quantification of indel percentage for the CACNA1D site. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; *** P < 0.001; **** P < 0.0001) were determined using a Student’s t -test. ( F ) Quantification of the editing efficiency when AcrIIA11 or AcrIIA4 are present relative to when no Acr is expressed. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; **** P < 0.0001) were determined using a Student’s t -test.
    Cmv Promoter Driven Acriia4 Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cmv promoter driven acriia4 expression vectors/product/Addgene inc
    Average 93 stars, based on 7 article reviews
    cmv promoter driven acriia4 expression vectors - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Mechanism of Cas9 inhibition by AcrIIA11"

    Article Title: Mechanism of Cas9 inhibition by AcrIIA11

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkaf318

    AcrIIA11 inhibits SaCas9 in vitro and in human HEK293T cells. ( A ) Agarose gel and ( B ) quantification of DNA cleavage by SaCas9. Graphs represent the mean of three replicates. Error bars: S.E.M. ( C ) HEK293Ts are transiently transfected with plasmids carrying SaCas9 + sgRNA. A second plasmid encodes either AcrIIA4 (positive control) or AcrIIA11. ( D ) Representative agarose gel and ( E ) quantification of indel percentage for the CACNA1D site. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; *** P < 0.001; **** P < 0.0001) were determined using a Student’s t -test. ( F ) Quantification of the editing efficiency when AcrIIA11 or AcrIIA4 are present relative to when no Acr is expressed. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; **** P < 0.0001) were determined using a Student’s t -test.
    Figure Legend Snippet: AcrIIA11 inhibits SaCas9 in vitro and in human HEK293T cells. ( A ) Agarose gel and ( B ) quantification of DNA cleavage by SaCas9. Graphs represent the mean of three replicates. Error bars: S.E.M. ( C ) HEK293Ts are transiently transfected with plasmids carrying SaCas9 + sgRNA. A second plasmid encodes either AcrIIA4 (positive control) or AcrIIA11. ( D ) Representative agarose gel and ( E ) quantification of indel percentage for the CACNA1D site. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; *** P < 0.001; **** P < 0.0001) were determined using a Student’s t -test. ( F ) Quantification of the editing efficiency when AcrIIA11 or AcrIIA4 are present relative to when no Acr is expressed. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; **** P < 0.0001) were determined using a Student’s t -test.

    Techniques Used: In Vitro, Agarose Gel Electrophoresis, Transfection, Plasmid Preparation, Positive Control, Standard Deviation



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    cmv promoter driven acriia4 expression vectors  (Addgene inc)
    93
    Addgene inc cmv promoter driven acriia4 expression vectors
    AcrIIA11 inhibits SaCas9 in vitro and in human HEK293T cells. ( A ) Agarose gel and ( B ) quantification of DNA cleavage by SaCas9. Graphs represent the mean of three replicates. Error bars: S.E.M. ( C ) HEK293Ts are transiently transfected with plasmids carrying SaCas9 + sgRNA. A second plasmid encodes either <t>AcrIIA4</t> (positive control) or AcrIIA11. ( D ) Representative agarose gel and ( E ) quantification of indel percentage for the CACNA1D site. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; *** P < 0.001; **** P < 0.0001) were determined using a Student’s t -test. ( F ) Quantification of the editing efficiency when AcrIIA11 or AcrIIA4 are present relative to when no Acr is expressed. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; **** P < 0.0001) were determined using a Student’s t -test.
    Cmv Promoter Driven Acriia4 Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cmv promoter driven acriia4 expression vectors/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    cmv promoter driven acriia4 expression vectors - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    AcrIIA11 inhibits SaCas9 in vitro and in human HEK293T cells. ( A ) Agarose gel and ( B ) quantification of DNA cleavage by SaCas9. Graphs represent the mean of three replicates. Error bars: S.E.M. ( C ) HEK293Ts are transiently transfected with plasmids carrying SaCas9 + sgRNA. A second plasmid encodes either AcrIIA4 (positive control) or AcrIIA11. ( D ) Representative agarose gel and ( E ) quantification of indel percentage for the CACNA1D site. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; *** P < 0.001; **** P < 0.0001) were determined using a Student’s t -test. ( F ) Quantification of the editing efficiency when AcrIIA11 or AcrIIA4 are present relative to when no Acr is expressed. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; **** P < 0.0001) were determined using a Student’s t -test.

    Journal: Nucleic Acids Research

    Article Title: Mechanism of Cas9 inhibition by AcrIIA11

    doi: 10.1093/nar/gkaf318

    Figure Lengend Snippet: AcrIIA11 inhibits SaCas9 in vitro and in human HEK293T cells. ( A ) Agarose gel and ( B ) quantification of DNA cleavage by SaCas9. Graphs represent the mean of three replicates. Error bars: S.E.M. ( C ) HEK293Ts are transiently transfected with plasmids carrying SaCas9 + sgRNA. A second plasmid encodes either AcrIIA4 (positive control) or AcrIIA11. ( D ) Representative agarose gel and ( E ) quantification of indel percentage for the CACNA1D site. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; *** P < 0.001; **** P < 0.0001) were determined using a Student’s t -test. ( F ) Quantification of the editing efficiency when AcrIIA11 or AcrIIA4 are present relative to when no Acr is expressed. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; **** P < 0.0001) were determined using a Student’s t -test.

    Article Snippet: The Sa Cas9 and CMV promoter-driven AcrIIA4 expression vectors were purchased from Addgene (Plasmid #85452 and #113038) [ , ].

    Techniques: In Vitro, Agarose Gel Electrophoresis, Transfection, Plasmid Preparation, Positive Control, Standard Deviation